Inverted terminal repeats (ITRs) of Adeno-associated virus (AAV) play a key role in virus replication and encapsidation, and are also important in viral genome integration and excision from the host genome. However, due to their high GC content and secondary structures, AAV plasmid ITRs are frequently mutated during plasmid production in E. coli., reducing experiment reproducibility and causing delays if ITR mutations are left undetected.
In this presentation, Dr. Elizabeth Louie from GENEWIZ will discuss our novel Adeno-Associated virus (AAV) plasmid DNA sequencing (RUO/GLP), preparation and synthesis solutions for rAAV production while confirming, maintaining and correcting inverted terminal repeat (ITR) regions. In addition, she will also talk about GENEWIZ’s proprietary Sanger & NGS viral genome sequencing confirmation solutions post AAV virus packaging including at the regulatory level to optimize AAV production for gene therapy development.